Fluorometer UFluo ONE II
Combined with a quantitative kit, the Ufluo ONE fluorometer uses fluorescent dyes to selectively bind to specific target molecules, which is more accurate than traditional UV absorption. Since traditional UV absorption is not selective and will measure the absorbance of all substances at 260nm - such as DNA, RNA, proteins, degraded nucleic acids and free nucleotides or excess salt ions. This product is a highly sensitive fluorometer specifically designed to detect concentration ranges lower than the detection limit of a micro-UV spectrophotometer.
Product Features
Application direction
Working process
Application field
Product parameters
Easy-to-use operation interface

(1) 7-inch high resolution LCD touch screen

(2) Several languages available: Chinese and English;

(3) Data export flexibility: support USB data transmission, easy data transmission and processing

(4) Measurement data can directly export to Excel for futher analysis

(5) Various testing programs can be preset, single plate can be tested for a number of different inspection items.

Intelligent component design meets the needs of various experiments

1) Equipped with two channels, with the ability to quickly analyze two kinds of fluorescence signals of a sample;

(2) A variety of optical channels are optional, suitable for different ssDNA and dsDNA, RNA, protein detection experiments;

(3) Open system: In addition to the standard reagents, it can also match most of the fluorescent detection reagents on the market; The sample tube is replaceable and can be selected in different sizes (0.5mL/0.2mL);

(4) Fast detection, fluorescence detection can be completed within 3 seconds

Application direction

Fluorometers are especially suitable for:

(1) Your sample is very rare and difficult to process

(2) Extracted dsDNA, oligos, RNA or a very small amount of protein

(3) The sample will be used in expensive downstream experiments: qPCR, PCR cloning, transfection and next-generation sequencing and other precision assays

Working process
Application field
  • 360 Excitation wavelength
    Nucleic acid quantification, plant GUS reporter gene detection, β-galactosidase activity detection, cell apoptosis detection
  • 470 Excitation Wavelength
    DNA, RNA quantification, green fluorescent protein GFP, gene detection, fluorescein detection, protein quantification, bacterial proliferation detection, Pgp activity detection, multidrug resistance (MDR)
  • 525 Excitation Wavelength
    Red fluorescent protein RFP gene detection, rhodamine detection, enzyme activity detection, cell activity toxicity detection, Cy-3 fluorescent labeling detection
  • 625 Excitation Wavelength
    RNA quantification, Cy-5 fluorescent labeling detection
360 Excitation wavelength
Nucleic acid quantification, plant GUS reporter gene detection, β-galactosidase activity detection, cell apoptosis detection
470 Excitation Wavelength
DNA, RNA quantification, green fluorescent protein GFP, gene detection, fluorescein detection, protein quantification, bacterial proliferation detection, Pgp activity detection, multidrug resistance (MDR)
525 Excitation Wavelength
Red fluorescent protein RFP gene detection, rhodamine detection, enzyme activity detection, cell activity toxicity detection, Cy-3 fluorescent labeling detection
625 Excitation Wavelength
RNA quantification, Cy-5 fluorescent labeling detection
Product parameters
Performance Features
  • Light source
    LED
  • Dynamic range
    5 orders of magnitude
  • Measurement speed
    <3s(single time)
Standard channel
  • Channel
    Excitation wavelength
    Emission wavelength
  • Blue
    470nm
    525nm
  • RED
    625nm
    690nm
Optional channel
  • Channel
    Excitation wavelength
    Emission wavelength
  • UV
    365nm
    460nm
  • GREEN
    525nm
    620nm
Common reagents and application
  • Excitation wavelength
    Commonly used fluorescent reagents
    Applications
  • 365
    Hoechst33258, 4-MU,EnZCheK Caspase
    Plant GUS report gene detection, cell apoptosis detection
  • 470
    PicoGreen®, oligreen,RiboGreen®, GFP, Protein, Fluorescein, Quant-iT™
    DsDNA,ssDNA quantitative, green fluorescent protein (GFP), fluorescent detection, protein quantitative
  • 525
    Rhodamine, Cy3, RFP Vybrant Cytotoxicity
    Rhodamine detection, Cy-3 fluorescence labeling detection, red fluorescent protein (RFP) gene detection, cell activity toxicity detection
  • 625
    Cy5, Quant-iT RNA
    Cy-5 fluorescence labeling detection, RNA quantitative